The 1927 Melbourne Carnival was the sixth edition of the Australian National Football Carnival, an Australian rules football interstate competition.
New South Wales caused the biggest upset of the carnival when they defeated Tasmania by three points and they also came close to beating Western Australia. The VFL once again finished on top of the table.
| Winning team | Score | Losing team | Score |
| Western Australia | 12.15 (87) | South Australia | 9.18 (72) |
| New South Wales | 12.11 (83) | Tasmania | 11.14 (80) |
| VFL | 21.19 (145) | South Australia | 14.12 (96) |
| Western Australia | 12.16 (88) | Tasmania | 12.15 (87) |
| VFL | 24.10 (154) | New South Wales | 9.12 (66) |
| South Australia | 20.23 (143) | Tasmania | 14.24 (108) |
| Western Australia | 18.14 (122) | New South Wales | 14.19 (103) |
| VFL | 24.11 (155) | Tasmania | 13.12 (90) |
| South Australia | 29.20 (194) | New South Wales | 6.11 (47) |
| VFL | 11.19 (85) | Western Australia | 10.12 (72) |